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cloned plasmids containing synthetic strs  (GenScript corporation)

 
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    GenScript corporation cloned plasmids containing synthetic strs
    The <t>synthetic</t> <t>STR</t> experiment summary. ( A ) Schematic description of the synthetic library. In each plasmid, a different synthetic STR construct was designed, synthesized and clone-sequenced for various STR types and length. The STR was designed within a context of an Illumina Truseq-HT dual index library to enable for nested PCR amplification at two time points (T 2 - amplification using outer primers only, T 3 -amplification using inner primers followed amplification by outer primers). The library is flanked by BsrDI restriction sites to enable direct sequencing of the STR library without amplification (T 1 ). Internal barcode (yellow triangle) is a short sequence, unique to each STR length to detect for cross-contamination. See text and methods for elaboration and for the designed constructs. ( B ) AC <t>STRs</t> repeat-number histograms, as were interpreted from sequencing results (T 1 , T 2 and T 3 ), compared to their expected length, T 0 (designed sequence). ( C ) Sequencing analysis results of each STR type, repeat-number and time point described as the percentage of the original (designed) signal from all the reads. Dashed line at the 5% marks the lower threshold of analysis: data points below the mark were deemed too noisy and were excluded from downstream analysis.
    Cloned Plasmids Containing Synthetic Strs, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cloned plasmids containing synthetic strs/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    cloned plasmids containing synthetic strs - by Bioz Stars, 2026-04
    90/100 stars

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    1) Product Images from "Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise"

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gky1318

    The synthetic STR experiment summary. ( A ) Schematic description of the synthetic library. In each plasmid, a different synthetic STR construct was designed, synthesized and clone-sequenced for various STR types and length. The STR was designed within a context of an Illumina Truseq-HT dual index library to enable for nested PCR amplification at two time points (T 2 - amplification using outer primers only, T 3 -amplification using inner primers followed amplification by outer primers). The library is flanked by BsrDI restriction sites to enable direct sequencing of the STR library without amplification (T 1 ). Internal barcode (yellow triangle) is a short sequence, unique to each STR length to detect for cross-contamination. See text and methods for elaboration and for the designed constructs. ( B ) AC STRs repeat-number histograms, as were interpreted from sequencing results (T 1 , T 2 and T 3 ), compared to their expected length, T 0 (designed sequence). ( C ) Sequencing analysis results of each STR type, repeat-number and time point described as the percentage of the original (designed) signal from all the reads. Dashed line at the 5% marks the lower threshold of analysis: data points below the mark were deemed too noisy and were excluded from downstream analysis.
    Figure Legend Snippet: The synthetic STR experiment summary. ( A ) Schematic description of the synthetic library. In each plasmid, a different synthetic STR construct was designed, synthesized and clone-sequenced for various STR types and length. The STR was designed within a context of an Illumina Truseq-HT dual index library to enable for nested PCR amplification at two time points (T 2 - amplification using outer primers only, T 3 -amplification using inner primers followed amplification by outer primers). The library is flanked by BsrDI restriction sites to enable direct sequencing of the STR library without amplification (T 1 ). Internal barcode (yellow triangle) is a short sequence, unique to each STR length to detect for cross-contamination. See text and methods for elaboration and for the designed constructs. ( B ) AC STRs repeat-number histograms, as were interpreted from sequencing results (T 1 , T 2 and T 3 ), compared to their expected length, T 0 (designed sequence). ( C ) Sequencing analysis results of each STR type, repeat-number and time point described as the percentage of the original (designed) signal from all the reads. Dashed line at the 5% marks the lower threshold of analysis: data points below the mark were deemed too noisy and were excluded from downstream analysis.

    Techniques Used: Plasmid Preparation, Construct, Synthesized, Nested PCR, Amplification, Sequencing



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    cloned plasmids containing synthetic strs  (GenScript corporation)
    90
    GenScript corporation cloned plasmids containing synthetic strs
    The <t>synthetic</t> <t>STR</t> experiment summary. ( A ) Schematic description of the synthetic library. In each plasmid, a different synthetic STR construct was designed, synthesized and clone-sequenced for various STR types and length. The STR was designed within a context of an Illumina Truseq-HT dual index library to enable for nested PCR amplification at two time points (T 2 - amplification using outer primers only, T 3 -amplification using inner primers followed amplification by outer primers). The library is flanked by BsrDI restriction sites to enable direct sequencing of the STR library without amplification (T 1 ). Internal barcode (yellow triangle) is a short sequence, unique to each STR length to detect for cross-contamination. See text and methods for elaboration and for the designed constructs. ( B ) AC <t>STRs</t> repeat-number histograms, as were interpreted from sequencing results (T 1 , T 2 and T 3 ), compared to their expected length, T 0 (designed sequence). ( C ) Sequencing analysis results of each STR type, repeat-number and time point described as the percentage of the original (designed) signal from all the reads. Dashed line at the 5% marks the lower threshold of analysis: data points below the mark were deemed too noisy and were excluded from downstream analysis.
    Cloned Plasmids Containing Synthetic Strs, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cloned plasmids containing synthetic strs/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    cloned plasmids containing synthetic strs - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The synthetic STR experiment summary. ( A ) Schematic description of the synthetic library. In each plasmid, a different synthetic STR construct was designed, synthesized and clone-sequenced for various STR types and length. The STR was designed within a context of an Illumina Truseq-HT dual index library to enable for nested PCR amplification at two time points (T 2 - amplification using outer primers only, T 3 -amplification using inner primers followed amplification by outer primers). The library is flanked by BsrDI restriction sites to enable direct sequencing of the STR library without amplification (T 1 ). Internal barcode (yellow triangle) is a short sequence, unique to each STR length to detect for cross-contamination. See text and methods for elaboration and for the designed constructs. ( B ) AC STRs repeat-number histograms, as were interpreted from sequencing results (T 1 , T 2 and T 3 ), compared to their expected length, T 0 (designed sequence). ( C ) Sequencing analysis results of each STR type, repeat-number and time point described as the percentage of the original (designed) signal from all the reads. Dashed line at the 5% marks the lower threshold of analysis: data points below the mark were deemed too noisy and were excluded from downstream analysis.

    Journal: Nucleic Acids Research

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise

    doi: 10.1093/nar/gky1318

    Figure Lengend Snippet: The synthetic STR experiment summary. ( A ) Schematic description of the synthetic library. In each plasmid, a different synthetic STR construct was designed, synthesized and clone-sequenced for various STR types and length. The STR was designed within a context of an Illumina Truseq-HT dual index library to enable for nested PCR amplification at two time points (T 2 - amplification using outer primers only, T 3 -amplification using inner primers followed amplification by outer primers). The library is flanked by BsrDI restriction sites to enable direct sequencing of the STR library without amplification (T 1 ). Internal barcode (yellow triangle) is a short sequence, unique to each STR length to detect for cross-contamination. See text and methods for elaboration and for the designed constructs. ( B ) AC STRs repeat-number histograms, as were interpreted from sequencing results (T 1 , T 2 and T 3 ), compared to their expected length, T 0 (designed sequence). ( C ) Sequencing analysis results of each STR type, repeat-number and time point described as the percentage of the original (designed) signal from all the reads. Dashed line at the 5% marks the lower threshold of analysis: data points below the mark were deemed too noisy and were excluded from downstream analysis.

    Article Snippet: STR plasmid design: Sequence verified cloned plasmids containing synthetic STRs of different types and sizes ( ) were ordered from either IDT or GenScript (pIDT-kan and modified puc57-Kan vectors, respectively).

    Techniques: Plasmid Preparation, Construct, Synthesized, Nested PCR, Amplification, Sequencing